mouse anti human integrin β6 antibody  (Bio-Techne corporation)

Journal: Frontiers in Nuclear Medicine

Article Title: αvβ6-integrin targeted PET/CT imaging in pancreatic cancer patients using 68 Ga-Trivehexin

doi: 10.3389/fnume.2024.1487602

Figure Lengend Snippet: Examples for ITGB6 (β-6-integrin) immunohistochemistry (IHC) with corresponding PDAC-scores (a) PDAC-score 1; (b) PDAC-score 2; (c) PDAC-score 3.

Article Snippet: Human tissue was immunohistochemically examined using an anti-human Integrin β6 (ITGB6) mouse monoclonal antibody [clone 442.5C4] (#407317, dilution 1:100, Merck Millipore, Burlington, Massachusetts, USA).

Techniques: Immunohistochemistry

Journal: Cancer Cell International

Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680

doi: 10.1186/s12935-024-03417-y

Figure Lengend Snippet: Analysis of αVβ6 expression. ( A ) ITGB6 proteomic expression profile in normal tissue and primary tumor (data from UALCAN) ( n = 71–108; *** p < 0.001; Two sample t test). ( B ) RT-qPCR analysis of ITGB6 mRNA expression; β-actin was used as housekeeping gene. The (αVβ6+) cell line HT-29 was used as reference ( n = 3–8; *** p < 0.001; One-way ANOVA followed by Tukey’s assay). ( C - D ) β6 integrin subunit protein expression assessed by Western-Blot; tubulin was used as a loading control ( n = 3–7; *** p < 0.001; One way ANOVA followed by Tukey’s assay). Data are presented as mean ± SEM

Article Snippet: 40 µg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 mouse anti-human β6 integrin subunit antibody (Merck Millipore corp, USA, 407,317) or 1:1000 mouse anti-α-tubulin antibody (Santa Cruz Biotechnology, 23,948), followed by incubation with 1:2000 anti-mouse IgG HRP-conjugated secondary antibody (Cell signaling technology, 7076 S) for 1 h at room temperature.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Cancer Cell International

Article Title: Targeting of 3D oral cancer spheroids by αVβ6 integrin using near-infrared peptide-conjugated IRDye 680

doi: 10.1186/s12935-024-03417-y

Figure Lengend Snippet: Multicellular tumor spheroid characterization. ( A ) Typical cryosection images of H10 and H5M5 spheroids after KI-67 immunohistochemistry staining (scale bar = 150 μm). ( B ) Typical immunofluorescence images of H10 and H5M5 spheroids cryosections stained with antibodies against αVβ6 integrin, cytokeratin 19, E-cadherin, vimentin, and fibronectin at day 4 post-seeding. Proteins of interest are in red and nuclei are in bleu after counterstaining with Vectashield-DAPI (scale bar = 100 μm at x40 and 40 μm at x100). ( C ) Relative quantification of immunofluorescence markers expression using ImageJ software ( n = 3–4; * p < 0.05, ** p < 0.01; Two sample t test). Data are presented as mean ± SEM

Article Snippet: 40 µg of protein lysate was loaded to 7.5% non-reducing SDS PAGE and after 150 min of migration and 90 min of blotting, PVDF membrane was saturated with 5% (w/v) solution of non-fat powered milk in TBST (Tris buffer solution with 0.1% Tween-20) for 1 h. Membrane was incubated overnight at 4 °C with either 1:100 mouse anti-human β6 integrin subunit antibody (Merck Millipore corp, USA, 407,317) or 1:1000 mouse anti-α-tubulin antibody (Santa Cruz Biotechnology, 23,948), followed by incubation with 1:2000 anti-mouse IgG HRP-conjugated secondary antibody (Cell signaling technology, 7076 S) for 1 h at room temperature.

Techniques: Immunohistochemistry, Staining, Immunofluorescence, Expressing, Software